SPRI beads can be used in a variety of NGS library prep chemistries and are compatible with both manual and automated processing. Their non-specific and reversible binding properties give scientists a tool for predictable and consistent size selection with high recovery of amplicons > 100 bp.
Ampure Xp Manual
For Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsDownstream applicationCloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern BlottingElution volume15 µL or aboveStarting materialDNA or RNA: PCR products, gDNA, cDNAStarting amountScalableDNA recovered>90% recovery for DNA >100 bpProcessing modeAutomated; manualThroughput96-384 samples per runDNA binding technologyMagnetic beadsStorage2C - 8CSpecial noteSize selection by varying beads ratioProtocol and ResourcesProduct Documentation & Literature
Agencourt AMPure XP magnetic beads (Beckman Coulter) are an efficient way to clean up samples for PCR, NGS, cloning and microarrays. The kit provides a solution for medium to high throughput requirements when carried out in a 96 well plate, but the protocol involves many washing and transfer steps that make it tedious to perform manually. With the VIAFLO 96, a handheld 96 channel electronic pipette, multistep protocols such as PCR clean-up and DNA purification can be performed quickly and efficiently, increasing throughput tremendously by transferring samples and reagents to all 96 wells at once. Thanks to its unique operating concept, the VIAFLO 96 remains as easy to use as a traditional handheld pipette and can even provide critical information (user-defined prompts) about the protocol steps.
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.
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